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GENTAUR NEWS 2009
Cholera Toxin
Cholera toxin has been reported to cause a variety of
effects on several different cell types. Depending on the cell type it can
induce, enhance or inhibit the cell proliferation, cell growth, cell
migration, immunization or cytokine and protein production. Cholera toxin
has proven to be an essential component in Molecular Biological Research and
an indispensable supplement for cell culture.
The naturally occurring cholera toxin belongs to the AB
class of bacterial toxins. It consists of a pentameric B oligomer that binds
to GM-1 receptors (e.g. on the surface of intestinal epithelial cells), and
an enzymatically active A subunit that is responsible for the toxicity of
the Vibrio cholerae bacterium.
GENTAUR
offers you both the complete cholera toxin isolated from
Vibrio cholerae
Inaba 569B and
the recombinant cholera toxin B-subunit.
The isolated cholera toxin (CTX) is delivered as 1 mg
lyophilized powder. No azide was used. Each vial, when reconstituted to 1.0
ml with sterile distilled water, contains 1.0 mg of protein in: 0.05 M Tris,
0.2 M NaCl, 0.001 M Na2EDTA at pH 7.5. Solubility of cholera toxin, in
water, decreases with time, following lyophilization. The CTX is delivered
chilled and should be stored at 4°C.
The recombinant cholera toxin B-subunit (rCTB) consists
only of the non-toxic part of the cholera enterotoxin. The protein is
completely free from the A-subunit, which has been genetically deleted from
the production strain. rCTB is fermented without antibiotics to ensure
safety. rCTB is delivered as a sterile solution in phosphate buffered saline
(PBS). No conservation additives are used. The protein concentration usually
ranges between 10-15 mg/ml. The rCTB solution is delivered chilled.
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