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GENTAUR NEWS 2009
EvaGreen qPCR Master Mix
Fast
EvaGreen® qPCR Master Mix
is a PCR reagent suitable for both qPCR using a fast cycling protocol and
high resolution melt curve (HRM) analysis. The reagent system uses two of
our breakthrough PCR technologies, EvaGreen® qPCR dye and
CheetahTM
Taq hotstart DNA polymerase, to deliver superior
results when compared to other commercial master mixes.
EvaGreen® Dye:
EvaGreen DNA-binding dye is the next generation dye
from Biotium for both real-time PCR detection and HRM. The dye selectively
binds to dsDNA via a novel "release-on-demand" mechanism that ensures low
PCR inhibition and permits HRM application at below saturation dye
concentration. Because the dye is spectrally similar to FAM or SYBR® Green
I, the dye is compatible with all commercial qPCR instruments. Moreover,
unlike SYBR® Green I and SYBR GreenER®, EvaGreen® dye is extremely stable.
Finally, EvaGreen® dye is nonmutagenic and noncytotoxic.
CheetahTM
Taq:
CheetahTM Taq is a new, chemically
modified hotstart DNA polymerase. Unlike Amplitaq Gold®, CheetahTM
Taq can be activated in as little as two minutes under
standard hotstart condition (i.e., 94°C in pH 8-9 Tris) with high activity
recovery. Also unlike antibody-based hotstart polymerases, such as Platinum®
Taq, CheetahTM Taq is completely
inactive at room temperature and has intrinsically low DNA contamination. In
addition, CheetahTM Taq is generally
more stable at -20°C or 4°C than AmpliTaq Gold®, ensuring that the master
mix maintain its peak performance following storage at low temperature.
Features:
·
Superior Sensitivity and wide dynamic range.
·
Suitable for both qPCR and HRM
·
Compatible with both fast and regular cycling protocols
·
Chemically-modified hotstart Taq requiring only 2 minutes to activate
·
Suitable for Taqman® assay
Code |
Product Name |
Quantity |
Price |
Fast EvaGreen®
M. Mix for qPCR and HRM (200 rxn) |
2x 1 ml € 155 |
€
155 |
|
Fast EvaGreen®
M. Mix for qPCR and HRM (500 rxn) |
5x 1 ml |
€
316 |
|
EvaGreen® 20X in
water |
5x 1 ml |
€
186 |
|
EvaGreen® qPCR
2x Basic Mix (for non-hotstart Taq) |
3x 1.7 ml |
€
186 |
|
EvaGreen® qPCR
2x Basic Mix HS (for hotstart Taq) |
3x 1.7 ml |
€
238 |
|
Cheetah Hot
Start Taq DNA Polymerase |
500 Units |
€
248 |
Check also, our other products here.
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