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GENTAUR NEWS 2009

 

EvaGreen qPCR Master Mix

Fast EvaGreen® qPCR Master Mix is a PCR reagent suitable for both qPCR using a fast cycling protocol and high resolution melt curve (HRM) analysis. The reagent system uses two of our breakthrough PCR technologies, EvaGreen® qPCR dye and Cheetah^TM Taq hotstart DNA polymerase, to deliver superior results when compared to other commercial master mixes.

EvaGreen® Dye: EvaGreen DNA-binding dye is the next generation dye from Biotium for both real-time PCR detection and HRM. The dye selectively binds to dsDNA via a novel "release-on-demand" mechanism that ensures low PCR inhibition and permits HRM application at below saturation dye concentration. Because the dye is spectrally similar to FAM or SYBR® Green I, the dye is compatible with all commercial qPCR instruments. Moreover, unlike SYBR® Green I and SYBR GreenER®, EvaGreen® dye is extremely stable. Finally, EvaGreen® dye is nonmutagenic and noncytotoxic.

Cheetah^TM Taq: Cheetah^TM Taq is a new, chemically modified hotstart DNA polymerase. Unlike Amplitaq Gold®, Cheetah^TM Taq can be activated in as little as two minutes under standard hotstart condition (i.e., 94°C in pH 8-9 Tris) with high activity recovery. Also unlike antibody-based hotstart polymerases, such as Platinum® Taq, Cheetah^TM Taq is completely inactive at room temperature and has intrinsically low DNA contamination. In addition, Cheetah^TM Taq is generally more stable at -20°C or 4°C than AmpliTaq Gold®, ensuring that the master mix maintain its peak performance following storage at low temperature.

 

Features:

·     Superior Sensitivity and wide dynamic range.

·     Suitable for both qPCR and HRM

·     Compatible with both fast and regular cycling protocols

·     Chemically-modified hotstart Taq requiring only 2 minutes to activate

·     Suitable for Taqman® assay

 

Code	Product Name	Quantity	Price
31003	Fast EvaGreen® M. Mix for qPCR and HRM (200 rxn)	2x 1 ml € 155	€ 155
31003-1	Fast EvaGreen® M. Mix for qPCR and HRM (500 rxn)	5x 1 ml	€ 316
31000	EvaGreen® 20X in water	5x 1 ml	€ 186
31001	EvaGreen® qPCR 2x Basic Mix (for non-hotstart Taq)	3x 1.7 ml	€ 186
31004	EvaGreen® qPCR 2x Basic Mix HS (for hotstart Taq)	3x 1.7 ml	€ 238
29050	Cheetah Hot Start Taq DNA Polymerase	500 Units	€ 248

 

 

Check also, our other products here.

 

 

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Copyright 2009 Gentaur
Last modified: 05/27/09